Text Box: Bioligand – macromolecule intermolecular interactions studied by spectroscopic and calorimetric techniques  
Text Box: National  Institute for R&D of Isotopic and Molecular Technologies Cluj-Napoca

Project financed by UEFISCDI


 PN II Program - IDEI

Project Type : Exploratory Research Project

Project code:  PN–II–ID-PCE-2011-3-0032

Contract nr.:  8/05.10.2011

Project span: 2011-2014


Project leader: Dr.  Mircea Bogdan


Project Abstract:

The study of intermolecular interactions in solutions is of central importance to most chemical and biochemical disciplines. The solution behavior of bio and macromolecules, their aggregation or intermolecular interactions is relevant for their biological activity. The binding affinity and the molecular recognition capacity can be described by the dissociation constant and the number of binding sites on the macromolecule. Any interaction that interferes with the binding of a bio-molecule to its receptor, such as competitive binding, may affect the pharmacological activity. The purpose of this project consist in the characterization of the molecular binding process between bio-ligands and receptors, using a variety of spectroscopic (NMR, UV/vis and fluorescence) and calorimetric techniques (ITC). Moreover, we intend to investigate the competitive binding process of two ligands to a macromolecular receptor and of two receptors to a bio-ligand. NMR is the major technique used to analyze the interaction between a bio-ligand and a receptor giving useful information about the, stoichiometry, stability, specific and non-specific binding sites. ITC is one of the most powerful and precise technique currently available to characterize the energetics of intermolecular interactions. It allows the binding functions ΔG, ΔH and ΔS to be determined within a single experiment and an accurate determination of the reaction stoichiometry and the dissociation constant.

Donat str, 67-103

Phone: 004-0264-584037  interior 181

Fax: 004-0264-420042

E-mail: mircea.bogdan@itim-cj.ro



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