Year

Goals

Activities

 2011

Autocomplexation of bioligands in aqueous solution

1. Investigation of the auto-association process of bioligands by NMR in liquid phase

 2. Dissemination of the obtained results

2012

1. Determination of the complexing properties (stoichiometry, association constant and molecular geometry) of β-CD with NSAIDs

2. The binding of some bioligands to HSA , studied by steady state fluorescence, UV-vis and ITC

1. Characterizing the ciclodextrin- bioligand inclusion complexes by NMR in aqueous solution.

2. Bioligand – human serum albumin interaction characterized by fluorescence, UV-vis and ITC

3. Dissemination of the obtained results and publishing scientific papers in ISI journals

4.Update the webb page

2013

1. Implementing non-selective and selective proton NMR relaxation rate measurements

2. Proposing a competitive low-affinity binding model in order to dete4rmine the mutual and specific sites of two ligands on a macromolecule

1. Implementation of selective nuclear relaxation techniques in order to analyze intermolecular interactions

2.Developing theoretical models for competitive binding processes: one receptor and two ligands and one ligand and two receptors, respectively.

2014

1. Defining the ligand- receptor binding strength, based on the “affinity index” and the “normalized affinity index” using selective longitudinal relaxation rate measurements

2. Analysis of the  theoretical models concerning the competitive binding process of two ligands on a macromolecule

1.1 Deducing the theoretical expressions of the “affinity index” and “normalized affinity index”

1.2 Performing NMR titration experiments, function of the receptor concentration

2.1 Deducing the analytical expressions based on selective longitudinal relaxation time measurements in order to calculate the specific parameters which characterize the competitive binding process between two ligands and a macromolecule

2015

Checking the theoretical model of competitive binding of two ligands on a macromolecule

Performing NMR titration experiments of a ligand in the presence of another ligand and a macromolecule in order to determine the association constants and the number of binding sites.

2016

NMR study of the competitive binding process of a ligand in the presence of two receptors (HSA and β-CD)

1. Calculating the theoretical expressions which characterize the association process of a competitive binding of a ligand in the presence of two receptors.

2. Performing titration NMR measurements in order to determine the parameters which characterize the competitive binding process.