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In this project, we aim to develop an efficient and reliable method used to monitor intracellular proteins by Electron Paramagnetic Resonance (EPR) spectroscopy. For this purpose, we will create a cellular model expressing Green Fluorescence Protein (GFP) tagged with an array of metal-binding oligopeptides (MeBPep) designed to trap EPR-responsive metal ions, such as Mn(II) or Cu(II). This model will be used to develop EPR spectroscopy as a toolbox for comparative profiling.<\/p>\n
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Project abstractIn this project, we aim to develop an efficient and reliable method used to monitor intracellular proteins by Electron Paramagnetic Resonance (EPR) spectroscopy. For this purpose, we will create a cellular model expressing Green Fluorescence Protein (GFP) tagged with an array of metal-binding oligopeptides (MeBPep) designed to trap EPR-responsive metal ions, such as Mn(II) […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-70","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.itim-cj.ro\/PNCDI\/mebpepepr\/wp-json\/wp\/v2\/pages\/70","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.itim-cj.ro\/PNCDI\/mebpepepr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.itim-cj.ro\/PNCDI\/mebpepepr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.itim-cj.ro\/PNCDI\/mebpepepr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.itim-cj.ro\/PNCDI\/mebpepepr\/wp-json\/wp\/v2\/comments?post=70"}],"version-history":[{"count":2,"href":"https:\/\/www.itim-cj.ro\/PNCDI\/mebpepepr\/wp-json\/wp\/v2\/pages\/70\/revisions"}],"predecessor-version":[{"id":73,"href":"https:\/\/www.itim-cj.ro\/PNCDI\/mebpepepr\/wp-json\/wp\/v2\/pages\/70\/revisions\/73"}],"wp:attachment":[{"href":"https:\/\/www.itim-cj.ro\/PNCDI\/mebpepepr\/wp-json\/wp\/v2\/media?parent=70"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}