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[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ admin_label=”About Us” module_id=”abstract” _builder_version=”4.16″ _module_preset=”default” background_enable_color=”off” custom_margin=”||||false|false” custom_padding=”50px||9px||false|false” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row use_custom_gutter=”on” gutter_width=”2″ padding_top_bottom_link_1=”false” _builder_version=”4.16″ _module_preset=”default” background_color=”#003bde” use_background_color_gradient=”on” background_color_gradient_direction=”160deg” background_color_gradient_stops=”#ffffff 0%|#ffffff 100%” background_color_gradient_start=”#ffffff” background_color_gradient_end=”#ffffff” width=”100%” module_alignment=”center” custom_margin=”||20px||false|false” custom_padding=”5px|||||” border_style_all=”none” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.27.0″ _module_preset=”default” text_font=”|300|||||||” text_font_size=”18px” text_line_height=”1.6em” text_orientation=”center” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]<\/p>\n
Obiectivul acestei proiect a fost de a fabrica o platform\u0103 senzoristic\u0103 pe baz\u0103 de filme sub\u021biri de oxid de zinc (ZnO) \u0219i argint (Ag), cu elemente periodice la scar\u0103 nanometric\u0103, destinat\u0103 detec\u021biei markerilor specifici bolilor neurodegenerative.<\/p>\n
\u00cen prim\u0103 instan\u021b\u0103, a fost identificat substratul polimeric \u0219i flexibil cel mai compatibil \u0219i potrivit pentru depunerea prin tehnica cu abla\u021bie laser (PLD) a filmelor sub\u021biri de ZnO cu structur\u0103 granular\u0103 \u0219i de Ag, prin metoda de tip magnetron sputtering (MS), c\u00e2t \u0219i pentru transferul re\u021belelor de nanotrenches \u0219i nanopillars de pe matri\u021b\u0103. A fost optimizat \u0219i aplicat cu succes un protocol dedicat pentru fabricarea de nanostructuri periodice de tip nanotrenches folosind litografia de nanoimprint (NIL), rezult\u00e2nd dou\u0103 produse noi pe baz\u0103 de re\u021bele de nanotrenches \u0219i nanopillars acoperite cu filme sub\u021biri de ZnO \u0219i Ag depuse prin PLD \u0219i MS. A fost determinat\u0103 grosimea optim\u0103 a filmului de Ag \u0219i tipul potrivit de nanostructuri periodice pentru o limit\u0103 de detec\u021bie SERS c\u00e2t mai mic\u0103 la identificarea unei molecule analit (cristal violet) \u0219i a unui neuromarker (\u00een acest caz a fost utilizat\u0103 molecula de dopamin\u0103 DA), similar\u0103 cu concentra\u021bia lui \u00een mediul biologic. At\u00e2t cu nanoplatformele SERS cu ZnO@Ag pe baz\u0103 de nanotrenches, c\u00e2t \u0219i \u00een cazul celor pe baz\u0103 de nanopillars au fost detectate solu\u021biile de CV la o concentra\u021bie de ordinul de nanomolar \u0219i a fost calculat un factor analitic de amplificare de ordinul 106<\/sup>. Aceea\u0219i concentra\u021bie a fost detectat\u0103 \u0219i \u00een cazul neuromarkerului DA, doar cu\u00a0 nanoplatforma de ZnO@Ag 7 nm pe baz\u0103 de nanotrenches. Aceasta a fost utilizat\u0103 \u0219i \u00een experimentele SERS de detec\u021bie de DA \u00een fluide simulate \u0219i de probe biologice extrase din \u0219oricei s\u0103n\u0103to\u0219i \u0219i cu boala Parkinson (BP) indus\u0103. \u00cen cazul detec\u021biei SERS a DA \u00een lichid cerebrospinal artificial s-a ob\u021binut o limit\u0103 de detec\u021bie de 10 \u00b5M \u0219i s-a\u00a0 calculat un factor analitic de amplificare SERS de 7\u00d7104<\/sup>.<\/p>\n \u00cen final, au avut loc experimente de inducere a bolii Parkinson la \u0219oareci de laborator, prin administrarea neurotoxinei MPTP. Fragmente de cortex \u0219i \u021besut striat, extrase din \u0219oareci de laborator s\u0103n\u0103to\u0219i \u0219i cu BP indus\u0103 au fost detectate cu succes prin spectroscopie SERS \u0219i diferen\u021biate prin analiza componentelor principale, pun\u00e2nd \u00een eviden\u021b\u0103 capabilitatea spectroscopiei SERS ca instrument de diagnostic \u0219i diferen\u021biere a bolii Parkinson.<\/p>\n [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" Executive SummaryObiectivul acestei proiect a fost de a fabrica o platform\u0103 senzoristic\u0103 pe baz\u0103 de filme sub\u021biri de oxid de zinc (ZnO) \u0219i argint (Ag), cu elemente periodice la scar\u0103 nanometric\u0103, destinat\u0103 detec\u021biei markerilor specifici bolilor neurodegenerative. \u00cen prim\u0103 instan\u021b\u0103, a fost identificat substratul polimeric \u0219i flexibil cel mai compatibil \u0219i potrivit pentru depunerea prin […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-202","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.itim-cj.ro\/PNCDI\/nanedissers\/wp-json\/wp\/v2\/pages\/202","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.itim-cj.ro\/PNCDI\/nanedissers\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.itim-cj.ro\/PNCDI\/nanedissers\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.itim-cj.ro\/PNCDI\/nanedissers\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.itim-cj.ro\/PNCDI\/nanedissers\/wp-json\/wp\/v2\/comments?post=202"}],"version-history":[{"count":2,"href":"https:\/\/www.itim-cj.ro\/PNCDI\/nanedissers\/wp-json\/wp\/v2\/pages\/202\/revisions"}],"predecessor-version":[{"id":205,"href":"https:\/\/www.itim-cj.ro\/PNCDI\/nanedissers\/wp-json\/wp\/v2\/pages\/202\/revisions\/205"}],"wp:attachment":[{"href":"https:\/\/www.itim-cj.ro\/PNCDI\/nanedissers\/wp-json\/wp\/v2\/media?parent=202"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}