Project Code: PN-II-RU-TE-2014-4-0862
PATHOGENIC MICROORGANISMS RAPID DETECTION AND IDENTIFICATION USING HIGH SENSITIVE RAMAN SPECTROSCOPY
DETECŢIA ŞI IDENTIFICAREA RAPIDĂ A MICROORGANISMELOR PATOGENE FOLOSIND
SPECTROSCOPIA RAMAN ULTRASENZITIVĂ
Our work
Welcome to our site
Here is a short description of the project
The bio-sensing for bacteria detection is currently explored by using various sensing
materials and approaches but it is still a challenge to find an alternative to the
conventional protocols, based on microorganisms cultivation. This project proposal
provides an ultrasensitive and selective, but simple, rapid and inexpensive bacteria
detection and identification method by using a receptor-free and innovative
immobilization principle of the biomass. Raman spectroscopy, which combines the
selectivity of the method with the sensitivity of the surface-enhanced Raman scattering
effect (SERS), is used in correlation with Principle Component Analysis (PCA) in order
to develop biosensors for pathogenic microorganisms. Most common pathogenic
bacteria will be tested by applying an original immobilization protocol and the in situ
synthesis method of the SERS-active nanostructures. The specific molecular information
gathered from the spectroscopic data will be uploaded and analyzed by means of
advanced statistical methods, with the final goal of creating an appropriate protocol
which will be used on complex biological samples. The rapid pathogens identification
and differentiation between multiple species, down to strain level will be carried out by
means of SERS-PCA bio-sensing. Furthermore, the efficiency of multiple classes of
antibiotics on bacteria viability will be investigated by SERS in order to obtain rapid and
precise diagnostic and targeted treatment.