Project Code: PN-II-RU-TE-2014-4-0862
PATHOGENIC MICROORGANISMS RAPID DETECTION AND IDENTIFICATION USING HIGH SENSITIVE RAMAN SPECTROSCOPY
DETECŢIA ŞI IDENTIFICAREA RAPIDĂ A MICROORGANISMELOR PATOGENE FOLOSIND
SPECTROSCOPIA RAMAN ULTRASENZITIVĂ
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Here is a short description of the project
The bio-sensing for bacteria detection is currently explored by using various sensing materials and approaches but it is still a challenge to find an alternative to the conventional protocols, based on microorganisms cultivation. This project proposal provides an ultrasensitive and selective, but simple, rapid and inexpensive bacteria detection and identification method by using a receptor-free and innovative immobilization principle of the biomass. Raman spectroscopy, which combines the selectivity of the method with the sensitivity of the surface-enhanced Raman scattering effect (SERS), is used in correlation with Principle Component Analysis (PCA) in order to develop biosensors for pathogenic microorganisms. Most common pathogenic bacteria will be tested by applying an original immobilization protocol and the in situ synthesis method of the SERS-active nanostructures. The specific molecular information gathered from the spectroscopic data will be uploaded and analyzed by means of advanced statistical methods, with the final goal of creating an appropriate protocol which will be used on complex biological samples. The rapid pathogens identification and differentiation between multiple species, down to strain level will be carried out by means of SERS-PCA bio-sensing. Furthermore, the efficiency of multiple classes of antibiotics on bacteria viability will be investigated by SERS in order to obtain rapid and precise diagnostic and targeted treatment.