DETERMINATION OF COFFEINE BY LC/MS FROM DIFFERENT BRANDS OF COOLING DRINKS

Lorena Filip1, Vlase Laurian2, Ioana Mindrutau,3 Doina Miere1

Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Hatieganu”, Cluj-Napoca,
 1Department of Environmental Chemistry,
2Department of Pharmaceutical Technology and Biopharmaceutics,  
3Department of Physical Chemistry

A high performance liquid chromatography coupled with mass spectrometry method (LC/MS) for quantification of caffeine from cooling drinks was elaborated. Detection and quantification of caffeine was based on monitoring the protonated molecular ion abundance (m/z 194.9). The quantification was made using the external standard method. The calibration curve was made on range 0.26-26 µg/ml for caffeine with a correlation coefficient greater than 0.995. Due the detection and quantification parameters obtained, this analytical method is rapid, simple and specific. 

References
1.McDevittV L, Rodriguez A, Williams KR, Analysis of soft drinks: UV spectrophotometry, liquid chromatography, and capillary electrophoresis. J.Chem.Educ., 75, 625 – 629,1998
 2.Nakashima K, Inoue K,  Mayahara K et al. Use of 3-(1,8-naphthalimido)propyl-modified silyl silica gel as a stationary phase for the high-performance liquid chromatographic separation of purine derivatives. J.Chromatogr.A, 722,107 – 113, 1996
3.Galasko GTF, Furman KI, Alberts E. The caffeine content of non-alcoholic beverages Food Chem.Toxicol., 27, 49 – 51, 1989