AMINO ACIDS QUANTITATION IN BIOLOGICAL MEDIA BY GC-MS

Monica Culea

“Babes-Bolyai” University, Str. M. Kogalniceanu, Nr. 1, Cluj-Napoca, 400084, Romania

The development of simple and sensitive analytical methods for the determination of amino acids in different biological specimen samples involves some steps of purification by ion exchange extraction technique followed by derivatization and gas chromatography/mass spectrometry (GC/MS) analysis. Amino acids from different biological samples were derivatized in two steps to obtain trifluoroacetyl ester derivatives. Finally, the extracted analytes were detected by GC/MS, EI, scan or SIM modes. The addition of the appropriate internal standard before the preparation steps is very important for the quantitative work. The methods were validated by using amino acid standard samples. Some application studies are presented.

References:
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