Project abstract

In this project, we aim to develop an efficient and reliable method used to monitor intracellular proteins by Electron Paramagnetic Resonance (EPR) spectroscopy. For this purpose, we will create a cellular model expressing Green Fluorescence Protein (GFP) tagged with an array of metal-binding oligopeptides (MeBPep) designed to trap EPR-responsive metal ions, such as Mn(II) or Cu(II). This model will be used to develop EPR spectroscopy as a toolbox for comparative profiling.