Stage 1

Growth of plant groups in microwave field; determination of the optimal methods for extraction of the bioactive compounds pursued in the study

1.1. Preparation of system for microwave irradiation of the plants considered for the study
1.2. Selection of extraction methods for bioactive compounds analyzed from the seasoning plants considered for the study
To achieve this objective are taken into account the following activities:
1.1.1. Planting celery, parsley and dill. Preparation of the anechoic chambers for the microwave irradiation of the plants that will be studied with both WLAN and GSM band

1.1.2. Applying the stress factors by introducing the plants into the irradiation and reference chambers, and monitoring their development
1.1.3. Preparation and characterization of vitamin C extracts obtained from reference plants, using new methods and different extraction solvents
1.1.4. Preparation and characterization of essential oils extracts from reference plants, using various new methods of extraction and different solvents
1.1.5. Selection of the method extraction of vitamin C and essential oils from the studied aromatic plants

Conclusions
During this stage, for the determination of the optimal conditions for the vitamin C extraction, HPLC and electrochemical methods were used. Extraction agents used were water and various aqueous solutions with the concentration of 8% of the following acids: trichloroacetic acid, metaphosphoric acid, acetic acid.

In order to establish the extraction conditions for the essential oils, it were obtained extracts with different solvents and their mixtures (eg, hexane, ethyl ether, ethanol etc.), extracts which were then analyzed by GC-FID.
Extraction methods used were: maceration, ultrasonic extraction, extraction in microwave field.
Comparing the chromatograms and amperograms obtained it was found that:
- using the ultrasonic extraction method, 8% metaphosphoric acid showed the best extraction properties for the vitamin C;
- maceration was the best extraction method for the essential oils, followed by ultrasonic extraction;
- the best extraction solvent was ethyl ether: ethanol (1: 1, v / v) mixture, followed by hexane.